Dual Glo Luciferase Assay - Bubbles?
Hi all,
I've recently been performing the Dual Glo Luciferase assay from Promega and had varying results. My most consistent results occurred when I pipetted to mix the luciferase reagent (which is essentially a lysis buffer) and also pipetted to mix the stop & glo reagent. However, there were quite a few bubbles.
I brought this concern to promega and was suggested to just use a plate shaker at 500rpm.
I've done this today but my results were not even close to what they were before.
Do you think it could be due to just a bad day or should I just mix with the pipette and pop the bubbles with a sterile needle?
Picture of plate with bubbles
